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Karyotypic and mtDNA basedcharacterization of Malaysian water buffalo

In Malaysia, the domestic water buffaloes (Bubalus bubalis) are classified into the swamp and the murrah buffaloes. Identification of these buffaloes is usually made via their phenotypic appearances. This study characterizes the subspecies of water buffaloes using karyotype, molecular and phylogenetic analyses. Blood of 105 buffaloes, phenotypically identified as swamp, murrah and crossbred buffaloes were cultured, terminated and harvested using conventional karyotype protocol to determine the number of chromosomes. Then, the D-loop of mitochondrial DNA of 10 swamp, 6 crossbred and 4 murrah buffaloes which were identified earlier by karyotyping were used to construct a phylogenetic tree was constructed.

FieldValue
Bidang Penyelidikan
Bioteknologi
Objektif Sosioekonomi
Advanced Experimental and Applied Science
Penerbit
Lesen
License Not Specified
Tahap Akses Awam
Public
Modified
2020-01-08
Release Date
2019-12-05
Identifier
b2631fd7-86cc-40c7-913a-3ac2466ecc88
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Anyone with following link https://doi.org/10.1186/s12863-019-0741-0 with will be able to read this content