Metabolomics is often used to comprehensively elucidate the metabolites in organisms like seaweed. Amino acids hydrolysed from proteins and certain targeted metabolites in seaweed have been investigated. However, water-soluble metabolites like free amino acids, organic acids, and sugars have seldom been comprehensively analysed. Metabolomics are valuable tools for these studies, but they require optimisation of pre-treatment methodology. Here, we evaluated various pre-treatment drying and extraction methods for brown seaweed metabolomics. Three edible brown seaweeds (Cladosiphon okamuranus [Mozuku], Saccharina japonica [Kombu], and Undaria pinnatifida [Wakame]) were used. Freeze-drying and oven-drying at both 40 and 80 °C were investigated. Methanol-water extracts with and without chloroform were compared. Metabolites were evaluated and quantified using liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry. The results showed that metabolite profiling was determined mainly by seaweed species identity rather than pre-treatment method. Freeze-drying yielded higher metabolite concentrations than oven-drying at either 40 or 80 °C. The effects of extraction with and without chloroform on metabolite concentration varied with seaweed species.
Data dan Sumber
Field | Value |
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Bidang Penyelidikan | Bioteknologi |
Objektif Sosioekonomi | Advanced Experimental and Applied Science |
Penerbit | |
Lesen | License Not Specified |
Tahap Akses Awam | Public |
Modified | 2020-01-08 |
Release Date | 2019-12-05 |
Identifier | b545dcce-b724-49b6-8d05-8e56982dbde2 |
The online version of this article ( https://doi.org/10.1007/s10811-018-1614-z) contains supplementary material, which is available to authorized users.